Review



mouse monoclonal αv5  (Bio-Rad)


Bioz Verified Symbol Bio-Rad is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Bio-Rad mouse monoclonal αv5
    (A) Tethering assay. FBF-2 is tethered by λN22 binding to boxB hairpins in the reporter RNA. Modified from Aoki et al. 201891. (B) Above, location of gonadal arm (black box) within animal. Below, location of distal region (dotted box) within gonadal arm. Asterisk marks distal end. Germline stem cells (GSCs) reside at the most distal end of germline and their daughters begin differentiation as they move proximally. (C) Diagram of distal gonad, showing distance from the distal end in microns below and extents of abundant FBF-2 (black line) and lower FBF-2 (dotted line) above. (D-E) Representative z-projections of extruded germlines. 20μm scale bar in (D) applies to all images. Dotted line marks gonad boundary; asterisk marks distal end. Top, GFP reporter expression (green); bottom, FBF-2FLAG staining (magenta). (D) Untethered wild-type FBF-2. (E) Tethered wild-type FBF-2. (F) ImageJ quantitation of reporter signal from tethered vs untethered FBF-2. GFP abundance plotted against distance from distal end. Solid line shows mean abundance and shading shows 95% confidence interval. Each plot represents three biological replicates with at least 10 gonad arms per replicate. P-values are given for pooled data in 0-35, 35-70 and 70-100 μm regions (black bars). P-values: *** p < 0.001, ** p < 0.01, * p < 0.05, ns (not significant) p > 0.05. Exact p-values in Table S4. (G-H) Representative z-projections of extruded germlines, as in (3D-E). (G) Untethered Y479A. (H) Tethered Y479A. (I) Quantitation of tethered vs untethered Y479A. P-values as in (F). (J) Western blots after FBF-2 and NTL-1 co-immunoprecipitation. Left, input lysates (1%); right, FLAG IP (10%). NTL-1:V5 co-immunoprecipitates with both wild-type and Y479A.
    Mouse Monoclonal αv5, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 787 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+%CE%B1v5/Mouse+anti+V5-Tag/pmc11253550-3-0-4
    Average 96 stars, based on 787 article reviews
    mouse monoclonal αv5 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "PUF partner interactions at a conserved interface shape the RNA binding landscape and cell fate in Caenorhabditis elegans"

    Article Title: PUF partner interactions at a conserved interface shape the RNA binding landscape and cell fate in Caenorhabditis elegans

    Journal: Developmental cell

    doi: 10.1016/j.devcel.2024.01.005

    (A) Tethering assay. FBF-2 is tethered by λN22 binding to boxB hairpins in the reporter RNA. Modified from Aoki et al. 201891. (B) Above, location of gonadal arm (black box) within animal. Below, location of distal region (dotted box) within gonadal arm. Asterisk marks distal end. Germline stem cells (GSCs) reside at the most distal end of germline and their daughters begin differentiation as they move proximally. (C) Diagram of distal gonad, showing distance from the distal end in microns below and extents of abundant FBF-2 (black line) and lower FBF-2 (dotted line) above. (D-E) Representative z-projections of extruded germlines. 20μm scale bar in (D) applies to all images. Dotted line marks gonad boundary; asterisk marks distal end. Top, GFP reporter expression (green); bottom, FBF-2FLAG staining (magenta). (D) Untethered wild-type FBF-2. (E) Tethered wild-type FBF-2. (F) ImageJ quantitation of reporter signal from tethered vs untethered FBF-2. GFP abundance plotted against distance from distal end. Solid line shows mean abundance and shading shows 95% confidence interval. Each plot represents three biological replicates with at least 10 gonad arms per replicate. P-values are given for pooled data in 0-35, 35-70 and 70-100 μm regions (black bars). P-values: *** p < 0.001, ** p < 0.01, * p < 0.05, ns (not significant) p > 0.05. Exact p-values in Table S4. (G-H) Representative z-projections of extruded germlines, as in (3D-E). (G) Untethered Y479A. (H) Tethered Y479A. (I) Quantitation of tethered vs untethered Y479A. P-values as in (F). (J) Western blots after FBF-2 and NTL-1 co-immunoprecipitation. Left, input lysates (1%); right, FLAG IP (10%). NTL-1:V5 co-immunoprecipitates with both wild-type and Y479A.
    Figure Legend Snippet: (A) Tethering assay. FBF-2 is tethered by λN22 binding to boxB hairpins in the reporter RNA. Modified from Aoki et al. 201891. (B) Above, location of gonadal arm (black box) within animal. Below, location of distal region (dotted box) within gonadal arm. Asterisk marks distal end. Germline stem cells (GSCs) reside at the most distal end of germline and their daughters begin differentiation as they move proximally. (C) Diagram of distal gonad, showing distance from the distal end in microns below and extents of abundant FBF-2 (black line) and lower FBF-2 (dotted line) above. (D-E) Representative z-projections of extruded germlines. 20μm scale bar in (D) applies to all images. Dotted line marks gonad boundary; asterisk marks distal end. Top, GFP reporter expression (green); bottom, FBF-2FLAG staining (magenta). (D) Untethered wild-type FBF-2. (E) Tethered wild-type FBF-2. (F) ImageJ quantitation of reporter signal from tethered vs untethered FBF-2. GFP abundance plotted against distance from distal end. Solid line shows mean abundance and shading shows 95% confidence interval. Each plot represents three biological replicates with at least 10 gonad arms per replicate. P-values are given for pooled data in 0-35, 35-70 and 70-100 μm regions (black bars). P-values: *** p < 0.001, ** p < 0.01, * p < 0.05, ns (not significant) p > 0.05. Exact p-values in Table S4. (G-H) Representative z-projections of extruded germlines, as in (3D-E). (G) Untethered Y479A. (H) Tethered Y479A. (I) Quantitation of tethered vs untethered Y479A. P-values as in (F). (J) Western blots after FBF-2 and NTL-1 co-immunoprecipitation. Left, input lysates (1%); right, FLAG IP (10%). NTL-1:V5 co-immunoprecipitates with both wild-type and Y479A.

    Techniques Used: Binding Assay, Modification, Expressing, Staining, Quantitation Assay, Western Blot, Immunoprecipitation

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Virus, Recombinant, Protease Inhibitor, Software, Sequencing

    Related Articles

    Binding Assay:

    Article Title: PUF partner interactions at a conserved interface shape the RNA binding landscape and cell fate in Caenorhabditis elegans
    Article Snippet: mouse monoclonal αV5 , Bio-Rad , Cat# MCA1360, RRID:AB_322378.

    Modification:

    Article Title: PUF partner interactions at a conserved interface shape the RNA binding landscape and cell fate in Caenorhabditis elegans
    Article Snippet: mouse monoclonal αV5 , Bio-Rad , Cat# MCA1360, RRID:AB_322378.

    Expressing:

    Article Title: PUF partner interactions at a conserved interface shape the RNA binding landscape and cell fate in Caenorhabditis elegans
    Article Snippet: mouse monoclonal αV5 , Bio-Rad , Cat# MCA1360, RRID:AB_322378.

    Staining:

    Article Title: PUF partner interactions at a conserved interface shape the RNA binding landscape and cell fate in Caenorhabditis elegans
    Article Snippet: mouse monoclonal αV5 , Bio-Rad , Cat# MCA1360, RRID:AB_322378.

    Quantitation Assay:

    Article Title: PUF partner interactions at a conserved interface shape the RNA binding landscape and cell fate in Caenorhabditis elegans
    Article Snippet: mouse monoclonal αV5 , Bio-Rad , Cat# MCA1360, RRID:AB_322378.

    Western Blot:

    Article Title: PUF partner interactions at a conserved interface shape the RNA binding landscape and cell fate in Caenorhabditis elegans
    Article Snippet: mouse monoclonal αV5 , Bio-Rad , Cat# MCA1360, RRID:AB_322378.

    Immunoprecipitation:

    Article Title: PUF partner interactions at a conserved interface shape the RNA binding landscape and cell fate in Caenorhabditis elegans
    Article Snippet: mouse monoclonal αV5 , Bio-Rad , Cat# MCA1360, RRID:AB_322378.

    Virus:

    Article Title: PUF partner interactions at a conserved interface shape the RNA binding landscape and cell fate in Caenorhabditis elegans
    Article Snippet: mouse monoclonal αV5 , Bio-Rad , Cat# MCA1360, RRID:AB_322378.

    Recombinant:

    Article Title: PUF partner interactions at a conserved interface shape the RNA binding landscape and cell fate in Caenorhabditis elegans
    Article Snippet: mouse monoclonal αV5 , Bio-Rad , Cat# MCA1360, RRID:AB_322378.

    Protease Inhibitor:

    Article Title: PUF partner interactions at a conserved interface shape the RNA binding landscape and cell fate in Caenorhabditis elegans
    Article Snippet: mouse monoclonal αV5 , Bio-Rad , Cat# MCA1360, RRID:AB_322378.

    Software:

    Article Title: PUF partner interactions at a conserved interface shape the RNA binding landscape and cell fate in Caenorhabditis elegans
    Article Snippet: mouse monoclonal αV5 , Bio-Rad , Cat# MCA1360, RRID:AB_322378.

    Sequencing:

    Article Title: PUF partner interactions at a conserved interface shape the RNA binding landscape and cell fate in Caenorhabditis elegans
    Article Snippet: mouse monoclonal αV5 , Bio-Rad , Cat# MCA1360, RRID:AB_322378.



    Similar Products

    mouse monoclonal αv5  (Bio-Rad)
    96
    Bio-Rad mouse monoclonal αv5
    (A) Tethering assay. FBF-2 is tethered by λN22 binding to boxB hairpins in the reporter RNA. Modified from Aoki et al. 201891. (B) Above, location of gonadal arm (black box) within animal. Below, location of distal region (dotted box) within gonadal arm. Asterisk marks distal end. Germline stem cells (GSCs) reside at the most distal end of germline and their daughters begin differentiation as they move proximally. (C) Diagram of distal gonad, showing distance from the distal end in microns below and extents of abundant FBF-2 (black line) and lower FBF-2 (dotted line) above. (D-E) Representative z-projections of extruded germlines. 20μm scale bar in (D) applies to all images. Dotted line marks gonad boundary; asterisk marks distal end. Top, GFP reporter expression (green); bottom, FBF-2FLAG staining (magenta). (D) Untethered wild-type FBF-2. (E) Tethered wild-type FBF-2. (F) ImageJ quantitation of reporter signal from tethered vs untethered FBF-2. GFP abundance plotted against distance from distal end. Solid line shows mean abundance and shading shows 95% confidence interval. Each plot represents three biological replicates with at least 10 gonad arms per replicate. P-values are given for pooled data in 0-35, 35-70 and 70-100 μm regions (black bars). P-values: *** p < 0.001, ** p < 0.01, * p < 0.05, ns (not significant) p > 0.05. Exact p-values in Table S4. (G-H) Representative z-projections of extruded germlines, as in (3D-E). (G) Untethered Y479A. (H) Tethered Y479A. (I) Quantitation of tethered vs untethered Y479A. P-values as in (F). (J) Western blots after FBF-2 and NTL-1 co-immunoprecipitation. Left, input lysates (1%); right, FLAG IP (10%). NTL-1:V5 co-immunoprecipitates with both wild-type and Y479A.
    Mouse Monoclonal αv5, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+%CE%B1v5/Mouse+anti+V5-Tag/pmc11253550-3-0-4
    Average 96 stars, based on 1 article reviews
    mouse monoclonal αv5 - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    mouse monoclonal αv5  (Thermo Fisher)
    90
    Thermo Fisher mouse monoclonal αv5

    Mouse Monoclonal αv5, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+%CE%B1v5/pmc06386581-7-0-4
    Average 90 stars, based on 1 article reviews
    mouse monoclonal αv5 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier
    mouse monoclonal αv5 antibody  (Thermo Fisher)
    90
    Thermo Fisher mouse monoclonal αv5 antibody

    Mouse Monoclonal αv5 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+%CE%B1v5/anti+v5/pmc06204812-452-0-23
    Average 90 stars, based on 1 article reviews
    mouse monoclonal αv5 antibody - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Tethering assay. FBF-2 is tethered by λN22 binding to boxB hairpins in the reporter RNA. Modified from Aoki et al. 201891. (B) Above, location of gonadal arm (black box) within animal. Below, location of distal region (dotted box) within gonadal arm. Asterisk marks distal end. Germline stem cells (GSCs) reside at the most distal end of germline and their daughters begin differentiation as they move proximally. (C) Diagram of distal gonad, showing distance from the distal end in microns below and extents of abundant FBF-2 (black line) and lower FBF-2 (dotted line) above. (D-E) Representative z-projections of extruded germlines. 20μm scale bar in (D) applies to all images. Dotted line marks gonad boundary; asterisk marks distal end. Top, GFP reporter expression (green); bottom, FBF-2FLAG staining (magenta). (D) Untethered wild-type FBF-2. (E) Tethered wild-type FBF-2. (F) ImageJ quantitation of reporter signal from tethered vs untethered FBF-2. GFP abundance plotted against distance from distal end. Solid line shows mean abundance and shading shows 95% confidence interval. Each plot represents three biological replicates with at least 10 gonad arms per replicate. P-values are given for pooled data in 0-35, 35-70 and 70-100 μm regions (black bars). P-values: *** p < 0.001, ** p < 0.01, * p < 0.05, ns (not significant) p > 0.05. Exact p-values in Table S4. (G-H) Representative z-projections of extruded germlines, as in (3D-E). (G) Untethered Y479A. (H) Tethered Y479A. (I) Quantitation of tethered vs untethered Y479A. P-values as in (F). (J) Western blots after FBF-2 and NTL-1 co-immunoprecipitation. Left, input lysates (1%); right, FLAG IP (10%). NTL-1:V5 co-immunoprecipitates with both wild-type and Y479A.

    Journal: Developmental cell

    Article Title: PUF partner interactions at a conserved interface shape the RNA binding landscape and cell fate in Caenorhabditis elegans

    doi: 10.1016/j.devcel.2024.01.005

    Figure Lengend Snippet: (A) Tethering assay. FBF-2 is tethered by λN22 binding to boxB hairpins in the reporter RNA. Modified from Aoki et al. 201891. (B) Above, location of gonadal arm (black box) within animal. Below, location of distal region (dotted box) within gonadal arm. Asterisk marks distal end. Germline stem cells (GSCs) reside at the most distal end of germline and their daughters begin differentiation as they move proximally. (C) Diagram of distal gonad, showing distance from the distal end in microns below and extents of abundant FBF-2 (black line) and lower FBF-2 (dotted line) above. (D-E) Representative z-projections of extruded germlines. 20μm scale bar in (D) applies to all images. Dotted line marks gonad boundary; asterisk marks distal end. Top, GFP reporter expression (green); bottom, FBF-2FLAG staining (magenta). (D) Untethered wild-type FBF-2. (E) Tethered wild-type FBF-2. (F) ImageJ quantitation of reporter signal from tethered vs untethered FBF-2. GFP abundance plotted against distance from distal end. Solid line shows mean abundance and shading shows 95% confidence interval. Each plot represents three biological replicates with at least 10 gonad arms per replicate. P-values are given for pooled data in 0-35, 35-70 and 70-100 μm regions (black bars). P-values: *** p < 0.001, ** p < 0.01, * p < 0.05, ns (not significant) p > 0.05. Exact p-values in Table S4. (G-H) Representative z-projections of extruded germlines, as in (3D-E). (G) Untethered Y479A. (H) Tethered Y479A. (I) Quantitation of tethered vs untethered Y479A. P-values as in (F). (J) Western blots after FBF-2 and NTL-1 co-immunoprecipitation. Left, input lysates (1%); right, FLAG IP (10%). NTL-1:V5 co-immunoprecipitates with both wild-type and Y479A.

    Article Snippet: mouse monoclonal αV5 , Bio-Rad , Cat# MCA1360, RRID:AB_322378.

    Techniques: Binding Assay, Modification, Expressing, Staining, Quantitation Assay, Western Blot, Immunoprecipitation

    KEY RESOURCES TABLE

    Journal: Developmental cell

    Article Title: PUF partner interactions at a conserved interface shape the RNA binding landscape and cell fate in Caenorhabditis elegans

    doi: 10.1016/j.devcel.2024.01.005

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: mouse monoclonal αV5 , Bio-Rad , Cat# MCA1360, RRID:AB_322378.

    Techniques: Virus, Recombinant, Protease Inhibitor, Software, Sequencing

    Journal: Cell

    Article Title: Excessive Cell Growth Causes Cytoplasm Dilution And Contributes to Senescence

    doi: 10.1016/j.cell.2019.01.018

    Figure Lengend Snippet:

    Article Snippet: Mouse monoclonal αV5 , Life Technologies , Cat# R960-25; RRID: AB_2556564.

    Techniques: Purification, Recombinant, Protease Inhibitor, Staining, Labeling, Expressing, Microarray, Bradford Protein Assay, Imaging, Plasmid Preparation, Software